Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 16
Abstract
A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.
Shown: Icos −/− T cells are impaired in pseudopod dynamics and persistent motility at the T-B border in the lymph node
GFP-expressing Icos +/+ (green) and dsRed-expressing Icos −/− (red) OT-2 T cells were imaged at the same T-B border. The image sequence was played twice (version a and b). (a) Regular 20 frames per second replay. (b) The depolarized state of both Icos +/+ (4 out of 81, ~5%) and Icos −/− T cells (12 out of 51, ~24%) is marked on six equally spaced time frames, exactly as used in the quantitation procedure described in the method.
Original publication: Xu H, Li X, Liu D, Li J, Zhang X, et al. 2013. Follicular T-helper cell recruitment governed by bystander B cells and ICOS-driven motility. Nature 496:523–27